Structural Insights into the Catalytic Mechanism of Phosphate Ester Hydrolysis by dUTPase
نویسندگان
چکیده
منابع مشابه
Structural insights into the catalytic mechanism of phosphate ester hydrolysis by dUTPase.
dUTPase is essential to keep uracil out of DNA. Crystal structures of substrate (dUTP and alpha,beta-imino-dUTP) and product complexes of wild type and mutant dUTPases were determined to reveal how an enzyme responsible for DNA integrity functions. A kinetic analysis of wild type and mutant dUTPases was performed to obtain relevant mechanistic information in solution. Substrate hydrolysis is sh...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2004
ISSN: 0021-9258
DOI: 10.1074/jbc.m406135200